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Dissection of the three vastus muscles was performed at an average distance of 12 mm ± 3 mm measured from the apex of the greater trochanter, with an average femur length in the evaluated rats of 40 mm ± 5 mm, after which muscle retraction was observed distal to the iliac bone and distal and anterior to the femoral bone, with the appearance of a large defect between the dissected ends (Figure 1)