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The FOXO4 peptide in a DRI conformation (D-amino acids in a retro-reversed sequence), henceforth named FOXO4-DRI, competes with FOXO4 for p53 binding in a dose-dependent manner and doing so with higher affinity.

Histological analysis of the intestinal barrier by light microscopy using hematoxylin and eosin (H&E) staining allow us to evaluate tissue integrity, the presence of mucosal ulcerations, and inflammatory infiltrate that could contribute to an increase in permeability [45]

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Similarly, to compare the relative effects of multiple NNMT inhibitors on NNMT activity in cultured adipocytes, differentiated adipocytes in 6-well plates were treated with 10 μM test compound for 24 h