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The proteins that make up the cellular architecture of the TJ and secure its connection to the actin cytoskeleton provide the structural integrity for the junctional complex to regulate transportation of solutes into the brain (Fig

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Western blotting Sample preparation Liver tissues were pulverized in liquid nitrogen and 20 mg were weighed and homogenized in ice cold 1X RIPA buffer (50 mM Tris, 150 mM sodium chloride (NaCl), 1% NP40, 0.5% sodium deoxycholate and 0.1% SD, pH 8) using a glass Dounce homogenizer