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PCR reaction mixture (total 10 µl) contained 0.5 µl of template cDNA, 0.2 µl each of the forward and reverse primers, 5 µl of FastStart Universal SYBR-Green Master Rox (Roche Diagnostics, Indianapolis, IN, USA), 0.2 µl Passive Reference Dye (Roche Diagnostics, USA) and 3.9 µl of H 2 O

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PMID 24748564