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Immunofluorescent microscopy analysis in HEK293T cells Micro Coverglass #1.5 coverslips (Electron Microscopy Sciences) coverslips were washed in 2 M HCl overnight at 55 °C, washed 5 times in sterile H 2 O, then pre-coated with 300 μL poly-D-lysine (10 μg/mL) overnight at 37 °C, then washed with distilled water three times and air-dried at room temperature for at least 2 h or stored at 4 °C until required
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For instance, they may play an important role in protein S-nitrosylation (S-NO)
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