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The modification can include the introduction, deletion, or substitution of one or more nucleotides at a target sequence of a polynucleotide of one or more cell(s)
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Total RNA was extracted from the mouse kidney and HK-2 cells using a column-type animal tissue total RNA extraction and purification kit (Sangon Biotech Co., Ltd.), and then reverse transcribed to synthesize complementary DNA using a PrimeScript™ RT Reagent Kit with gDNA Eraser Kit (Takara Bio, Inc.)
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