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Cells were fixed with 4% PFA in PBS at room temperature for 15 min, permeabilized with 1% saponin and stained by rabbit anti-ACP1 antibody (LifeSpan Bioscience) followed by Alexa 488-conjugated goat anti-rabbit IgG antibody (Invitrogen)
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Studies have indicated that the increase of the autophagy level in vivo can lead to the degradation of ferritin and the increase of iron level, thus leading to oxidative damage of cells through the Fenton reaction [66, 67]
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