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Purified Ero1α (2 µM) and 0.04–400 µM ZnSO 4 were preincubated in Chelex-treated and air-saturated reaction buffer (20 mM HEPES-NaOH, pH 7.2, and 150 mM NaCl) at 30 °C for 10 min, and DTT (20 µM) was preincubated in Chelex-treated and degassed reaction buffer at 30 °C for 10 min in another tube

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21 TABLE 3 Medications That Should Be Avoided By Persons with G6PD Deficiency* TABLE 5 Symptoms and Laboratory Evaluation in Patients with G6PD and Acute Hemolysis Drugs that cause hemolysis in G6PD-deficient persons inf lict oxidative damage to erythrocytes leading to erythrocyte destruction