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Although the direct cytotoxic effects of high cellular GSSG levels have been documented in a variety of cell types 33,34,35,36 , our cell culture studies with hepatocytes revealed that increased GSSG accumulation, despite being non-toxic per se, could sensitize hepatocytes to TNFα-induced killing, suggesting that GSSG and TNFα can synergistically induce hepatocyte death and that GSSG-triggered sensitization to TNFα hepatotoxicity represents a clinically relevant process that may account for disease progression in NAFLD

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2025;16 doi: 10.3389/fimmu.2025.1608407