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When Lucy could no longer afford to procure street fentanyl, she hit a crisis point and presented to a health clinic in the throes of withdrawal, “so sick I was blacking out.” A nurse connected Lucy with Millar, at which time Lucy began taking 2 g of SA every 2 waking hours

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Here, we describe the GST-His method as a new small-scale affinity purification system for recombinant proteins, based on a N-terminal Glutathione Sepharose Tag (GST) 2,3 and a C-terminal 10xHis tag 4 , which are both fused to the protein of interest